GABA like receptors distribution in the central nervous system of chasmagnathus

CARBO TANO M; HEPP Y.; FREUDENTHAL RAMIRO; PEDREIRA MARIA EUGENIA

Huerta Grande Córdoba

Congreso; Segunda Reunión Conjunta en Neurociencias IIRCN; 2010

TAN Y SAN

Gamma-amino butyric acid (GABA) is the most abundant inhibitory neurotransmitter in the central nervous system (CNS). A considerable amount of evidence showed the role of the GABAA receptor in diverse behavioral paradigms. We have already demonstrated that compounds that enhance the action of GABA impair the consolidation and reconsolidation processes, and compounds that reduce the action of GABA have the opposite action on the context learning in the crab Chasmagnathus. Our objective is to study the differential role of the GABAérgic system in acquisition, consolidation, reconsolidation and extinction memory in this paradigm. In particular, we are interested in the plastic modification of the GABAergic system as consequence of the different memory processes. To achieve this objective it was necessary to characterize the GABAergic CNS in the crab. Here we show the first approaches in the study of the GABAérgic system using commercial antibodies raised against GABA and the mammalian beta 2/3 subunit of the GABAA receptor in immunohistochemistry experiments and western blot assays. Taken together, these results show that it would be possible to analyze the plastic modifications suffered at receptor level as a consequence of the different phases or memory processes.