IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
capítulos de libros
Título:
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
Autor/es:
CECILIA BORASSI; JULIANA PEREZ DI GIORGIO; MARÍA R. SCARPIN; JORGE MUSCHIETTI; JOSE ESTEVEZ
Libro:
Plant Cell Growth and Expansion. Series Methods in Molecular Biology
Editorial:
Springer Science+Business Media New York
Referencias:
Año: 2014; p. 41 - 47
Resumen:
Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 hours can reach up to 800 m. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed.