IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
capítulos de libros
Título:
Coupled promoter splicing systems
Autor/es:
MANUEL J. MUÑOZ, MANUEL DE LA MATA AND ALBERTO R. KORNBLIHTT
Libro:
RNA splicing: The complete guide
Editorial:
Wiley-Blackwell
Referencias:
Lugar: Weinheim, Germany. ; Año: 2012; p. 400 - 407
Resumen:
We report here the experimental strategies and details to assess alternative splicingin mammalian cells transfected with reporter minigenes carrying differentpromoters or whose transcription is carried our by different artificial mutants ofRNA polymerase II. These experiments were successfully used in our lab todemonstrate the coupling between transcription and alternative splicing and theroles of the carboxy terminal domain of RNA polymerase II in the recruitment ofsplicing factors and of the rate of transcriptional elongation on splice site selection.