IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
ci21A/Asr1 expression influences glucose accumulation
Autor/es:
NICOLÁS FRANKEL; ADRIANO NUNES-NESI; ILSE BALBO; JEANNINE MAZUCH; DANILO CENTENO; NORBERTO D. IUSEM; ALISDAIR R. FERNIE; FERNANDO CARRARI
Revista:
PLANT MOLECULAR BIOLOGY
Editorial:
Springer
Referencias:
Lugar: Amsterdam; Año: 2007 vol. 63 p. 719 - 730
ISSN:
0167-4412
Resumen:
Asr genes are exclusively found in the
genomes of higher plants. In many species, this gene
family is expressed under abiotic stress conditions and
during fruit ripening. The encoded proteins have
nuclear localisation and consequently a transcription
factor function has been suggested. Interestingly, yeastone-
hybrid experiments revealed that a grape ASR
binds to the promoter of a hexose transporter gene
(VvHT1). However, the role of these proteins in plantagenes are exclusively found in the
genomes of higher plants. In many species, this gene
family is expressed under abiotic stress conditions and
during fruit ripening. The encoded proteins have
nuclear localisation and consequently a transcription
factor function has been suggested. Interestingly, yeastone-
hybrid experiments revealed that a grape ASR
binds to the promoter of a hexose transporter gene
(VvHT1). However, the role of these proteins in plantaVvHT1). However, the role of these proteins in planta
is still elusive. By using a reverse genetics approach in
potato we found that modification of Asr1 expression
has no incidence on the aerial phenotype of the plant
but exerts a dramatic effect in tuber. Asr1 antisense
potatoes displayed decreased tuber fresh weight
whereas Asr1 overexpressors had a diminished number
of tubers. Moreover, overexpression lines showed lower
transcript levels of a plasma membrane hexose transporter
and a concomitant decrease in glucose content in
parenchyma cells of potato tubers. On the same hand
glucose uptake rate was also reduced in one of the
overexpressing lines. It thus seems likely that Asr1 is
involved in the control of hexose uptake in heterotrophic
organs. In addition, the transgenic plants were
characterized by several other changes in steady state
metabolite levels. Results presented here support a role
for ci21A/Asr1 in glucose metabolism of potato tuber.Asr1 expression
has no incidence on the aerial phenotype of the plant
but exerts a dramatic effect in tuber. Asr1 antisense
potatoes displayed decreased tuber fresh weight
whereas Asr1 overexpressors had a diminished number
of tubers. Moreover, overexpression lines showed lower
transcript levels of a plasma membrane hexose transporter
and a concomitant decrease in glucose content in
parenchyma cells of potato tubers. On the same hand
glucose uptake rate was also reduced in one of the
overexpressing lines. It thus seems likely that Asr1 is
involved in the control of hexose uptake in heterotrophic
organs. In addition, the transgenic plants were
characterized by several other changes in steady state
metabolite levels. Results presented here support a role
for ci21A/Asr1 in glucose metabolism of potato tuber.Asr1 antisense
potatoes displayed decreased tuber fresh weight
whereas Asr1 overexpressors had a diminished number
of tubers. Moreover, overexpression lines showed lower
transcript levels of a plasma membrane hexose transporter
and a concomitant decrease in glucose content in
parenchyma cells of potato tubers. On the same hand
glucose uptake rate was also reduced in one of the
overexpressing lines. It thus seems likely that Asr1 is
involved in the control of hexose uptake in heterotrophic
organs. In addition, the transgenic plants were
characterized by several other changes in steady state
metabolite levels. Results presented here support a role
for ci21A/Asr1 in glucose metabolism of potato tuber.Asr1 overexpressors had a diminished number
of tubers. Moreover, overexpression lines showed lower
transcript levels of a plasma membrane hexose transporter
and a concomitant decrease in glucose content in
parenchyma cells of potato tubers. On the same hand
glucose uptake rate was also reduced in one of the
overexpressing lines. It thus seems likely that Asr1 is
involved in the control of hexose uptake in heterotrophic
organs. In addition, the transgenic plants were
characterized by several other changes in steady state
metabolite levels. Results presented here support a role
for ci21A/Asr1 in glucose metabolism of potato tuber.Asr1 is
involved in the control of hexose uptake in heterotrophic
organs. In addition, the transgenic plants were
characterized by several other changes in steady state
metabolite levels. Results presented here support a role
for ci21A/Asr1 in glucose metabolism of potato tuber.ci21A/Asr1 in glucose metabolism of potato tuber.