IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
Apis mellifera octopamine receptor 1 (AmOA1) expression in antennal lobe networks of the honey bee (Apis mellifera) and fruit fly (Drosophila melanogaster).
Autor/es:
SINAKEVITCH, I; SMITH ADRIAN; FERNANDO LOCATELLI; HUERTA R.; MAXIM BAZHENOV; SMITH BRIAN
Revista:
Frontiers in Systems Neurosciences
Editorial:
Frontiers
Referencias:
Lugar: Lausanne Switzerland; Año: 2013 vol. 25 p. 7 - 70
ISSN:
1662-5137
Resumen:
Octopamine (OA) underlies reinforcement during appetitive conditioning in the honey beeand fruit fly, acting via different subtypes of receptors. Recently, antibodies raised againsta peptide sequence of one honey bee OA receptor, AmOA1, were used to study thedistribution of these receptors in the honey bee brain (Sinakevitch et al., 2011). Theseantibodies also recognize an isoform of the AmOA1 ortholog in the fruit fly (OAMB,mushroom body OA receptor). Here we describe in detail the distribution of AmOA1receptors in different types of neurons in the honey bee and fruit fly antennal lobes.We integrate this information into a detailed anatomical analysis of olfactory receptorneurons (ORNs), uni- and multi-glomerular projection neurons (uPNs, and mPNs) and localinterneurons (LNs) in glomeruli of the antennal lobe. These neurons were revealed by dyeinjection into the antennal nerve, antennal lobe, medial and lateral antenno-protocerbraltracts (m-APT and l-APT), and lateral protocerebral lobe (LPL) by use of labeled cell lines inthe fruit fly or by staining with anti-GABA.We found that ORN receptor terminals and uPNslargely do not show immunostaining for AmOA1. About seventeen GABAergic mPNs leavethe antennal lobe through the ml-APT and branch into the LPL. Many, but not all, mPNsshow staining for AmOA1. AmOA1 receptors are also in glomeruli on GABAergic processesassociated with LNs. The data suggest that in both species one important action of OA inthe antennal lobe involves modulation of different types of inhibitory neurons via AmOA1receptors. We integrated this new information into a model of circuitry within glomeruli ofthe antennal lobes of these species.