IFIBYNE 05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
Using Cell-ID 1.4 with R for Microscope-Based Cytometry
Autor/es:
ALAN BUSH; ARIEL CHERNOMORETZ; RICHARD YU; ANDREW GORDON; ALEJANDRO COLMAN-LERNER
Revista:
Current Protocols in Cell Biology
Editorial:
John Wiley & Sons, Current Protocols Customer Service
Referencias:
Lugar: Somerset, NJ 08875-5597 USA ; Año: 2012 vol. 99 p. 14181 - 141727
ISSN:
1934-2500
Resumen:
This unit describes a method for quantifying various cellular features (e.g., volume, totaland subcellular fluorescence localization) from sets of microscope images of individualcells. It includes procedures for tracking cells over time. One purposely defocusedtransmission image (sometimes referred to as bright-field or BF) is acquired to segmentthe image and locate each cell. Fluorescence images (one for each of the color channelsto be analyzed) are then acquired by conventional wide-field epifluorescence or confocalmicroscopy. This method uses the image-processing capabilities of Cell-ID and dataanalysis by the statistical programming framework R, which is supplemented with apackage of routines for analyzing Cell-ID output. Both Cell-ID and the analysis packageare open-source. Curr. Protoc. Mol. Biol. 100:14.18.1-14.18.26. C 2012 by John Wiley& Sons, Inc.
