IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
O-glycosylated cell wall proteins are essential in root hair growth
Autor/es:
VELASQUEZ SM, RICARDI MM, DOROSZ JG, FERNANDEZ PV, NADRA AD, POL-FACHIN L, EGELUND J, GILLE S, HARHOLT J, CIANCIA M, VERLI H, PAULY M, BACIC A, OLSEN CE, ULVSKOV P, PETERSEN BL, SOMERVILLE C, IUSEM ND, ESTEVEZ JM.
Revista:
SCIENCE
Editorial:
AMER ASSOC ADVANCEMENT SCIENCE
Referencias:
Año: 2011 p. 1401 - 1403
ISSN:
0036-8075
Resumen:
Root hairs are single cells that develop by tip growth and are
specialized in the absorption of nutrients. Their cell walls are
composed of polysaccharides and hydroxyproline-rich glycoproteins
(HRGPs) that include extensins (EXTs) and arabinogalactan-proteins
(AGPs). Proline hydroxylation, an early posttranslational modification
of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the
subsequent O-glycosylation sites in EXTs (which are mainly
arabinosylated) and AGPs (which are mainly arabinogalactosylated). We
explored the biological function of P4Hs, arabinosyltransferases, and
EXTs in root hair cell growth. Biochemical inhibition or genetic
disruption resulted in the blockage of polarized growth in root hairs
and reduced arabinosylation of EXTs. Our results demonstrate that
correct O-glycosylation on EXTs is essential for cell-wall self-assembly
and, hence, root hair elongation in Arabidopsis thaliana.