IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
artículos
Título:
Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
Autor/es:
SCHAAF, C.; SHAN, B.; ONOFRI, C.; STALLA, G.K.; ARZT, E.; SCHILLING, T.; PERONE, M.J.; RENNER, U.
Revista:
NEUROENDOCRINOLOGY
Editorial:
KARGER
Referencias:
Año: 2010 p. 200 - 210
ISSN:
0028-3835
Resumen:
The polyphenol curcumin (diferuloylmethane) is the active componenet of
the spice plant Curcuma longa and has been shown to exert multiple
actions on mammalian cells. We have studied its effect on
folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a
multifunctional, endocrine inactive cell type of the anterior
pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a
monolayer cell culture and in the colony formation assay in soft agar.
Fluorescence-activated cell-sorting (FACS) analysis demonstrated
curcumin-induced cell cycle arrest at G(2)/M accompanied by inhibition
of cyclin D(1) protein expression. Curcumin had a small effect on
necrosis of TtT/GF cells, but it mainly stimulated apoptosis as
demonstrated by FACS analysis (Annexin V-fluorescein
isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced
apoptosis involved suppression of Bcl-2, stimulation of cleaved
caspase-3 and induction of DNA fragmentation. Functional studies on FS
cell-derived compounds showed that curcumin inhibited mRNA synthesis and
release of angiogenic vascular endothelial growth factor-A (VEGF-A).
Immune-like functions of FS cells were impaired since curcumin
downregulated Toll-like receptor 4, reduced nuclear factor-kappaB
expression and suppressed bacterial endotoxin-induced interleukin-6
(IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6
production was also found in primary rat pituitary cell cultures, in
which FS cells are the only source of these proteins. The observed
effects of curcumin on FS cell growth, apoptosis and functions may have
therapeutic consequences for the intrapituitary regulation of hormone
production and release as well as for pituitary tumor pathogenesis.