IAFE   05512
INSTITUTO DE ASTRONOMIA Y FISICA DEL ESPACIO
Unidad Ejecutora - UE
artículos
Título:
Male and Female Reproductive Toxicity induced by moderate chronic ethanol exposure in CF-1 mice
Autor/es:
CEBRAL, E.; ABREVAYA, X.C.; MUDRY, M.D
Revista:
CELL BIOLOGY AND TOXICOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2011 vol. 27 p. 237 - 248
ISSN:
0742-2091
Resumen:
Since genetic damage induced by ethanol exposure is controversial and incompleteand because germ and somatic cells constitute bioindicators for monitoring reproductivetoxicity and genotoxic actions of ethanol consumption, the purpose of the presentinvestigation was to evaluate morphological sperm and oocyte alterations and parentalgenotoxic effects after chronic moderate ethanol intake in the CF-1 outbred mouse model.Ethanol 10% was administered to CF-1 adult male (treated males, TM) and female (treatedfemales, TF) mice for 27 days, whereas water was given to controls (CM and CF). Posttreatmentmicronucleus frequency (MN-PCE/1000/mouse) and gamete morphology wereevaluated. To test whether change of female reproductive status results in maternalgenotoxicity, CF-1 females received ethanol 10% (exposed group, PTF) or water (controlgroup, PCF) in drinking water for 17 days previous and up to 10 days of gestation. TM had ahigh percentage of abnormal spermatozoa vs CM (p<0.001) and elevated parthenogeneticactivated oocyte frequency appeared in TF vs CF (p<0.001). Chronic ethanol ingestion inducedincreased MN frequency in TM and TF (p<0.01). In periconceptionally treated females (PTF),where blood alcohol concentrations were between 19-28 mg/dl, very significantly increased MNfrequency was found vs PCF (p<0.01), whereas MN values were similar to TF. These resultsshow that chronic moderate alcohol ingestion in CF-1 mouse produces sperm headdysmorphogenesis and oocyte nuclear anomalies, suggesting that morphological abnormalities ingerm cells are probably related to parental genotoxicity after ethanol consumption.