EFFECT OF LOCAL ANAESTHETIC TOTALCAINE FORTE ON HEME METABOLISM AND ANTIOXIDANT DEFENSE SYSTEM IN A GENETIC MICE MODEL OF ACUTE INTERMITENT PORPHYRIA

SASHA JAZMÍN MIKULSKYJ; ANA MARIA BUZALEH; MARÍA DEL C. MARTINEZ; JOHANNA ROMINA ZUCCOLI,; TOBIAS CARABAJAL WALDHUTER

Milan

Congreso; International Congress on Porphyrins and Porphyrias; 2019

Universitá Digli Studi di Milano

Acute attacks of Porphyria may be precipitated by exogenous drugs including anaesthetics. There is limited information in the literature about the use of some dental medicines in the acute Porphyrias. The safety of local anaesthetic agents remains a controversial issue due to experimental evidence reveals that some of them are porphyrogenic in either animal models (lidocaine) or cell culture (lidocaine, prilocaine, bupivacaine), however clinical experience has shown that its use in patients with acute Porphyria had no notable adverse effect. Articaine (carticaine, methyl-4-methyl-3-(2-propylaminopropionamide)thiophene-2-carboxylate hydrochloride) Is a relatively new local anaesthetic and still untried in acute porphyria, although some reports established its safety and an others classified this drug as unsafe. The aim was to investigate the effect of a commercial preparation, Totalcaina Forte (carticaine chlorhidrate:L-adrenaline, Bernabo Laboratories) on heme metabolism in CF1 mice. Animals received different doses (1, 5, 7 mg/kg, s.c.) and were sacrificed at different times (30 and 60 minutes) after injection. 5-Aminolevulinic acid synthetase (ALA-S), Porphobilinogen deaminase (PBG-D) and Heme oxygenase (HO) were measured in different tissues. No variations of ALA-S specific activity was observed with any of the doses and times assayed. PBG-D activity was unchanged for 1 or 5 mg/kg of Totalcaine, independently of the tissue; although a significant reduction of liver activity (35%, p