Volatile Anaesthetics and Mitochondria Damage: Alterations on Chain Respiratory and Krebs Cycle.

MARTINEZ MARIA DEL CARMEN; BUZALEH ANA MARIA; RUSPINI, SILVINA; ZUCCOLI, JOHANNA; BATLLE, ALCIRA

Burdeos

Congreso; ICPP Porphyrins and Porphyrias 2017; 2017

Heme deficiency by a reduced synthesis or an accelerated catabolism would trigger severe cell damage. Previously we demonstrated that porphyrinogenic agents affected several brain metabolisms including respiratory mitochondrial chain in encephalon CF1 mice. The aim was to study the effects of volatile anaesthetics and other xenobiotics on the activity of I to IV complexes of respiratory chain in encephalon of a mouse model of Acute Intermittent Porphyria. Male knockout mice (PBGD-/-) were treated with Isoflurane (2 ml/kg), Sevoflurane (1,5 ml/kg), ethanol (30%), allylisopropylacetamide (AIA, 350 mg/kg) and Veronal (167 mg/kg), or starved 24 hours. Veronal increased the activity of II-III complexes (370%, p