DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES INTO BEIGE ADIPOCYTES CONTRIBUTE TO BREAST CANCER PROGRESSION

CECILIA LOTUFO; MARÍA FERNANDA RIERA; MARIANA GANTOV; GUSTAVO RINDONE; JUAN CARLOS CALVO; PRISCILA PAGNOTTA; MARÍA FERNANDA RIERA; JUDITH TONEATTO

Virtual

Congreso; Reunión anual de Biociencias (SAIC); 2020

Crosstalk between breast cancer cells and adipose tissue in the mammary microenvironment plays a key role in the regulation of tumor behavior. We previously demonstrated morphologic and metabolic changes in adipocytes indirectly cocultured with breast cancer cells, suggesting a first step towards browning of adipose tissue, which is an important contribution to the hypermetabolic state of breast cancer. Thus, the aim of study was to obtain insight into the effect of epithelial cell-beige adipocyte communication on tumor progression. Here, we characterized components present in con- ditioned media (CMs) from beige adipocytes (BA) or white adipo- cytes (WA) achieved upon differentiation of 3T3-L1 preadipocytes, and evaluated the effects of BA- and WA-CMs on both adhesion and migration of tumor (LM3, 4T1 and MC4-L1) and non-tumor (NMuMG) mouse mammary epithelial cell lines. Additionally, we analyzed the expression of ObR, CD44, vimentin, MMP-9, MCT1 and LDH on tumor and non-tumor mouse mammary epithelial cell lines incubated with BA-, WA-CMs or Ctrol-CMs (control). 3T3-L1 preadipocytes differentiated into beige adipocytes upon PPARg ac- tivation (rosiglitazone), displaying characteristics that morphologi- cally resembled brown/beige adipocytes. Levels of UCP1, CIDEA, GLUT4, leptin, MCT4 and FABP4 were increased while adiponectin, caveolin 1, and perilipin 1 levels were decreased in BA with respect to WA. Tumor cell lines revealed lower cell adhesion (p