CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
20-Hidroxyeicosatetranoic acid (20-HETE) modulates the transcriptional activity of the androgen receptor acting through the GPR75 receptor in androgen dependent prostate cancer cells
Autor/es:
MORMANDI E; NOWICKI S; CARDENAS S; DAKARAPU R; GUTIERREZ M; FALCK J
Lugar:
CABA
Reunión:
Congreso; LXIVI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2020
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
We have previously shown the role of 20-HETE, the product of 20-hydroxylation of arachidonic acid, in sustaining the viability of androgen- dependent prostate cancer cells (LNCaP) by acting as a signaling molecule downstream the androgen receptor (AR). Recently, the GPR75 receptor has been identified as the target for 20-HETE. The aim of this study was to identify the role of the 20-HETE/GPR75 axis in the AR transcriptional activity (ARTA) in LNCaP cells. The following methods were used: western blot (expression of GPR75); immunofluorescence (intracellular localization of AR); reporter gene assay (ARTA in LNCaP cells transfected with PSA-Luc or with an empty vector); chemiluminescence (prostate specific antigen (PSA) quantification). Results were analyzed using two tails t-student test, one-way ANOVA followed by Dunnet?s, or two ways ANOVA followed by Sidak?s.The expression of GPR75 was first confirmed in LNCaP cells. Both, 20-HETE (0.1nM, 45min) and dihydrotestosterone (DHT, 10nM, 30min) increased by 63%, the abundance of AR in the nuclei (p