CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Participation of SIRT1 in the regulation of mature Sertoli cell (SC) energy metabolism
Autor/es:
GORGA, AGOSTINA; PELLIZZARI, ELIANA HERMINIA; GALARDO, MARÍA NOEL; RINDONE, GUSTAVO MARCELO; CAMBEROS, MARÍA DEL CARMEN; MERONI, SILVINA BEATRIZ; CENTOLA, CECILIA LUCIA; RIERA, MARÍA FERNANDA
Lugar:
Modalidad virtual
Reunión:
Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
SCs provide structural and nutritional support for germ cells (GC) development. SC metabolism has particular characteristics. It converts glucose to lactate, the main energy substrate for GC, and uses fatty acids (FA) as its own energy source. SC is also capable of synthesizing triglycerides and storing them in lipid droplets (LD). In this context, the simultaneous regulation of lipid metabolism and lactate production may be relevant to the seminiferous tubule physiology. Sirtuins (SIRT1-7) belong to a NAD+-dependent enzymes family that act as cellular energy sensors. SIRT1, the most studied member, plays an important role in processes ranging from cell cycle regulation to energy homeostasis. The aim of this work was to evaluate the participation of SIRT1 in the regulation of lactate production and of FA metabolism in SCs. SC cultures obtained from 20-day-old rats were incubated in the absence (B) or presence of resveratrol 50 µM (RSV, SIRT1 activator). Results are expressed as X±SD of three independent experiments (*p