EFFECT OF CHEMOTHERAPY ON ENDOCRINE TESTICULAR FUNCTION DURING CHILHOOD AND PUBERTY IN PATIENTS WITH ACUTE LYMPHOBLASTIC LEUKEMIA OT NON-HODGKIN LYMPHOMA: A PROSPECTIVE, LONGITUDINAL STUDY

BRENZONI, LUCIANA; GUTIERREZ, MARIANA; RODRIGUEZ, MARIA EUGENIA; GAMBOA, MARÍA AROZARENA; BALLERINI, MARÍA GABRIELA; GRINSPON, ROMINA; PRADA, SILVINA; BEDECARRAS, PATRICIA; AVERSA, LUIS

Congreso; Reunión Anual de Sociedades de Biociencias; 2020

Introduction: Chemotherapy can have variable effects accordingto the proliferative state of cell populations. Testicular Sertoli cellsshow differences in proliferative status between infancy and puberty.Aim: To determine if there is a differential effect of chemotherapy onSertoli cell function between children and adolescents with hematologicmalignancies during and after treatment. Secondarily, testosteroneand gonadotropins were evaluated.Methods: prospective cohort study in children with acute lymphoblasticleukaemia or non-Hodgkin lymphoma. Serum levels of AMHand FSH (direct and indirect marker of Sertoli cells, respectively)and of testosterone (T) and LH (direct and indirect markers of Leydigcells) were evaluated during chemotherapy and for 24 monthsafter the end of the treatment. Results were analysed according topubertal stage and expressed as median (range).Results: 16 prepubertal boys, age 4.2 yr (0.7-7.1), and 5 pubertal,age 13.4 yr (11.7-14.8) were analysed. Follow-up was 4.6 ± 0.6years. In prepubertal boys, AMH was pre-treatment (tx): 709 pmol/L(331-1333), at 3 months (m) of tx: 1191 (396-1660), 6 m: 934 (273-1325), 12 m: 577 (338-1474), 24 m (end tx): 1156 (625-1563); inthe subsequent follow-up, 3 m: 1096 (532-1708), 12 m: 1212 (603-2601) and 24 m: 1011 (520-2035). 24 m after end of tx, serum AMHwas higher than baseline in all patients. T, FSH and LH were alwaysnormal. In pubertal boys, AMH was pre-tx 74 pmol/L (39-121), 3 m:76 (47-98), 6 m: 92 (64-173), 12 m: 74 (55-229), 24 m (end tx): 95(46-118); in follow-up: 3 m: 124 (37-174), 12 m: 140 (76-157) and24 m: 129 (32-173). T was always normal. FSH and LH increasedduring chemotherapy and normalised 24 m after the end of chemotherapy.Conclusion: In prepubertal boys, no compromise of Sertoli cellfunction was observed during the entire follow-up. In pubertal boys,a transient elevation of gonadotropins was observed, which may reflecta reversible deleterious effect of chemotherapy.