mTORC1 regulation of glycolytic metabolism in proliferating Sertoli cells (SC)

RINDONE GM; RIERA MF; CENTOLA CL; CAMBEROS MC; MERONI SB; GORGA A; PELLIZZARI EH; GALARDO MN

Mar del Plata

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

The final number of SC reached during the proliferative periods determines sperm production capacity in adulthood. FSH is the major Sertoli cell mitogen exerting its action by activation of cAMP/PKA, Erk1/2 and PI3K/Akt/mTORC1 dependent pathways. mTORC1 regulates a wide range of cellular functions, including metabolism and cell proliferation. On the other hand, changes in metabolism are proliferating cell hallmarks. Proliferating cells seem to rely on aerobic glycolysis in order to support anabolic reactions associated with cell cycle progression. Although mature SC metabolism was thoroughly evaluated, the metabolism regulation in proliferating SC has been neglected. The aim of this study was to analyze whether aerobic glycolysis is regulated by FSH through mTORC1 pathway. SC obtained from 8-day old rats were maintained under basal conditions (B) or stimulated with FSH in the absence or presence or rapamycin (Rap) -specific mTORC1 inhibitor. Lactate (Lac) -glycolytic flux marker- production and PFKFB3 expression by RT-qPCR were evaluated. Results are expressed as mean±SD of three independent experiments (different letters indicate statistically significant differences, P