CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of the oligosaccharides of FSH on the regulation of AMH promoter in prepubertal Sertoli cells.
Autor/es:
CAMPO S; HELENA SCHTEINGART; URRUTIA M,; REY RA
Lugar:
Mar del Plata
Reunión:
Conferencia; SAIC; 2019
Institución organizadora:
Sociedad Argentina de Investigacion Clinica
Resumen:
The relative proportion of different FSH glycosylation variants (FSH-GV) changes during sexual development. FSH regulates anti-Müllerian hormone (AMH) secretion by testicular Sertoli cells from fetal life until puberty.The objective of this work was to evaluate the role of FSH-GV in the regulation of the human AMH promoter in prepubertal Sertoli cells.To test this, recombinant human FSH (rhFSH) was separated by isoelectrofocusing to isolate FSH-GV with different degrees of sialylation: the acidic (AC) pH 3-4 and the basic (BA) pH5-7 variants. Alternatively, rhFSH was separated by Concavalin-A chromatography to isolate FSH-GV with different degrees of oligosaccharide complexity: unbound (UB) and weakly bound (WB). The concentration of each FSH-GV was determined by electrochemiluminescence immunoassay.Reporter assays were performed in the prepubertal murine SMAT1 Sertoli cell line co-transfected with a hFSH receptor expression plasmid and a firefly luciferase-associated AMH promoter plasmid. Luciferase was measured after incubation with the whole rhFSH or the FSH-GV fractions (20 ng/ml) for 24 hours. Results, expressed as relative luciferase units (RLU, mean ± SEM), were compared using ANOVA followed by Friedman post-test.