Participation of SIRT1 in the regulation of Sertoli cell proliferation

GORGA A; PELLIZZARI EH; GALARDO MN; REGUEIRA M; RIERA MF; RINDONE GM; CAMBEROS MC; MERONI SB

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica

Sertoli cells (SC) provide the structural and nutritional support for germ cell development. Considering that each SC is able to support a limited number of germ cells, the final number of SC reached during the proliferative periods determines sperm production capacity in adulthood. In the rat, SC proliferate during fetal and neonatal periods and it is well known that FSH is the major SC mitogen; however, little is known about the mechanisms involved in the detention of SC proliferation, essential for the formation of blood-testis barrier and SC differentiation.Sirtuins (SIRT1-7) belong to a cellular energy sensor family of NAD+-dependent enzymes with deacetylase activity. SIRT1, the most studied member, plays an important role in several processes ranging from cell cycle regulation to energy homeostasis. The aim of this work was to investigate whether SIRT1 activation participates in the cessation of SC proliferation. Mitotically active SC obtained from 8-day old rats were maintained under basal (B) conditions or stimulated with FSH 100 ng/ml in the absence or presence of Resveratrol (RSV, 50µM) a polyphenol found in grapes that increases SIRT1 activity. BrdU incorporation and the expression of cyclins D and of the cell cycle inhibitors p21 and p27 by RT-qPCR were evaluated. Results are expressed as mean±SD (n=3) (different letters indicated statistically significant differences P