Potential participation of PPAR gamma in FSH regulation of fatty acid storage in Sertoli cells

REGUEIRA MARIANA; GALARDO MARÍA NOEL; CIGORRAGA SELVA B; RINDONE GUSTAVO; CAMBEROS MARÍA DEL CAMEN; MERONI SILVINA BEATRIZ; GORGA AGOSTINA; PELLIZZARI ELIANA HERMINIA; RIERA MARIA FERNANDA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica

Sertoli cells (SC) are necessary to provide an adequate and protected environment for germ cell development. Studies of SC glucose metabolism have shown that SC actively metabolize glucose but that the vast majority is converted to lactate that represents the preferential energy substrate for germ cells. In this metabolic scenario, fatty acids (FA) have been demonstrated to be needed to sustain SC energy status. The presence of numerous lipid droplets (LD) is characteristic in SC and it is assumed that they constitute the storage of these FA. In this context, we have demonstrated that FSH the master hormone in the regulation of SC physiology? regulates LD content and the expression of the fatty acid transporter FAT/CD36 and of proteins that regulates LD formation and stability, PLIN1 and 2. On the other hand, we have also shown that pharmacological activation of PPARγ participates in the regulation of lipid storage in this cell type, although the participation of this transcription factor in hormonal regulation has not been analyzed yet in SC. The aim of this study was to analyze the potencial participation of PPARγ in FSH regulation of the expression of proteins involved in FA transport and storage. SC from 20-day old rats were used. Cultures were treated with FSH (100 ng/ml) in presence or absence of a PPARγ antagonist (T007; 50 µM). Results are expressed as mean±SD, n=3, different letters indicate statistically signifcant differences, p