CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression and direct role of CCR2 receptor and its chemokine ligands within the feline cumulus oocyte complex and the antral follicle wall.
Autor/es:
JAWORSKI, JP; PELUFFO, MC; ROJO, JL
Lugar:
CABA
Reunión:
Congreso; Reunión Conjunta de Biociencias 2017; 2017
Institución organizadora:
SAIC mas 9 sociedades
Resumen:
The aim of this study was to: 1) evaluate the mRNA expression of CCR2, its ligands (MCP1, MCP2, MCP3 and MCP4) and genes related with periovulatory events (AREG and HAS2) within the cumulus oocyte complex (COC) and follicle wall after LH stimulus using a feline antral follicle culture, and 2) evaluate the mRNA expression of CCR2, MCP1, AREG and HAS2 within the COC, using a feline COC culture in the presence of MCP1. First, ovaries were removed from adult domestic cats (n=52). Antral follicles larger than 0.5 mm were mechanically dissected from the ovary and individually cultured (n=130) for 6, 12, 24 or 36h with or without recombinant human LH (75 mIU/ml). At the end of the culture, COCs and follicle walls were dissected to further analyze the mRNA expression by qPCR. A subset of COCs (n=20) were fixed for CCR2 and MCP1 immunofluorescence. In a second experiment, COCs (n=28) were isolated from antral follicles (n=7 cats) and cultured for 3h (time when periovulatory gene expression peaks in our COC culture) with or without recombinant human MCP1 (10 and 100 ng/ml) and mRNA expression was assessed by qPCR. Two-way ANOVA analyses (Time-Treatment) showed significant effects of mRNA expression for many of the genes. LH treatment significantly increased (p