CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Quantitative targeted proteomics analysis of one-carbon metabolism proteins in human liver cancer
Autor/es:
CORRALES F; AMBAO V; SANGRO B; PARADELA A; GRANERO I
Reunión:
Congreso; XII EuPA Congress; 2018
Resumen:
Background: Primary liver cancer (HCC) is recognized as the fifth most common neoplasm and the second leading cause of cancer death worldwide. Although most risk factors are known, and the molecular pathogenesis has been widely studied the underlying molecular mechanisms remain to be unveiled. This is a central issue to facilitate the definition of novel biomarkers and clinical targets for more effective patient management. We utilize the B/D-HPP popular protein strategy to detect proteins that have been associated to liver cancer in previous studies. Several enzymes highlight the known metabolic remodeling of liver cancer cells, four of which participate in one-carbon metabolism (1CM). This pathway is central to the maintenance of differentiated hepatocytes, as it is considered the connection between intermediate metabolism and epigenetic regulation.Methods: We designed a targeted selective reaction monitoring (SRM) method to track quantitatively 15 different 1CM proteins in human liver samples (control, HCC and cirrhotic). This method allows systematic monitoring of one-carbon metabolism and could prove useful in the follow-up of HCC and of chronically liver-diseased patients (cirrhosis) at risk of HCC.Results: Relevant changes occur at the quantitative level when non-diseased and tumor liver samples are compared. A more complex quantitative pattern was found from samples obtained from cirrhotic livers, suggesting that these particular samples are more heterogeneous than previously expected.Conclusions: Significant and reliable quantitative changes found for several 1CM-specific proteins could be useful for the diagnosis and prognosis of human liver cancer.