Inhibition of Anti-Müllerian Hormone (AMH) Transcription By Androgens Requires the Presence of the Androgen Receptor and Intact SF1 Response Elements

EDELSZTEIN, N.; REY, R.; SCHTEINGART, H.F.; VALERI, C.

Boston

Congreso; 98th Annual Meeting of the Endocrine Society; 2016

Endocrine Society

Androgens bind to the androgen receptor (AR), which interacts with specific elements of target genes, thus regulating their expression. However, rapid non-genomic effects of androgens are now also accepted as contributing to the physiological effects of the steroids. In Sertoli cells, AMH expression is inhibited by androgens through an unknown mechanism.Here we tested whether androgens exert a direct effect on the AMH promoter activity, using luciferase assays in the pre-pubertal Sertoli cell line SMAT1, after transfection with the AR and length variants or mutants of the AMH promoter. Results are expressed as percentage (mean ± SEM), and compared against basal activity (theoretical value of 100%), using a one sample t-test. When SMAT1 cells transfected with an AR expression vector were treated with DHT (10-7M), a decrease in a [-3068 to -1] bp AMH promoter activity was observed, whether the treatment lasted 5 minutes (64.0 ± 6.0%, P = 0,002) or 24 hours (45.5 ± 2.8%; P