Characterization of Insulin Like Growth Factor (IGF) System Components in Pediatric Tumors of Central Nervous System (CNS)

VENARA MARCELA; MARTÍN, AYELEN; PENNISI PATRICIA A; GARCIA LOMBARDI, M; CLEMENT, FLORENCIA; BERGADA, I

Buenos Aires

Congreso; XXIV Reunión Anual de la Sociedad Latinoamericana de Endocrinología Pediátrica (SLEP)XXVI Reunión Anual de la Sociedad Latinoamericana de Endocrinología Pediátrica (SLEP); 2016

Sociedad Latinoamericana de Endocrinología Pediátrica (SLEP)

Background: CNS tumors are the most frequent solid tumors in pediatric population. The IGF system of ligands and receptors are known to play an important role in both normal and neoplastic growth. Recently, quantitation of components of this system have been reported in CNS tumors from adult population, but information from pediatric patients is scarce.Objective: To characterize the expression of IGF-1, IGF-1R, IGF-2 and IR in CNS tumors from pediatric patients.Patients and Methods: We performed a prospective study (6/2012-6/2016) of pediatric patients with CNS tumors without previous medical treatment that underwent surgery in our Hospital. Tissues were collected at the time of surgery. IGF-1, IGF-1R, IGF-2 and IR expression was measured by qRTPCR using extracted RNA. Specimens were classified according to localization or histology type and grade based on WHO2007 classification. Mann-Whitney, Kruskal-Wallis followed by Dunn´s Testwere used for comparisons.Results: We included 89 patients (50 males/39 females), median aged 8.9yrs, (range 0.9-18.6). The most common subgroups of CNS tumors were gliomas (n:36); ependymomas (n:16); medulloblastomas (n:11). Levels of IGF-1, IGF-1R, IGF-2 and IR mRNA were detectable in all specimens. Changes in IGF-2 mRNA levels among tumors were significantly different compared to the changes observed in the other genes analyzed (IGF-2:47(12-82) vs IGF-1:1(0.7-1.4); IGF-1R:0.8(0.5-1.0); IR:0.9(0.7-1.1),mean(CI) fold change, p