SF1 recognition sequences in the Anti-Mullerian hormone (AMH) gene promoter participate together with the androgen receptor (AR) in the inhibition caused by androgens

SCHTEINGART HELENA F; EDELSZTEIN, NADIA Y; REY RA

Buenos Aires

Congreso; XXVI Annual Meeting of the Latin American Pediatric Endocrinology Society (SLEP); 2016

SLEP

In pre-pubertal Sertoli cells, AMH expression is inhibited by androgens through an unknown mechanism. Upon ligand binding, the AR interacts with specific response elements and/or other transcription factors within the regulatory regions of its target genes. We tested whether androgens exert a direct effect on the AMH promoter activity, using luciferase assays in the pre-pubertal Sertoli cell line SMAT1, after transfection with an AR expression vector and length variants or mutants of the AMH promoter. Results are expressed as percentage (mean±SEM), and compared against basal activity (theoretical value of 100%), using a one sample t-test. Endogenous levels of AMH in response to androgens were also evaluated through RT-qPCR. In AR-transfected SMAT1 cells, a decrease was observed in a [-3068 to -1] bp AMH promoter activity with a 5 min (66.0±6.9%, P=0,008) or 24 h (46.0±3.0%; P