CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Estrogens Are Involved in the Activation of Anti-Müllerian Hormone (AMH) Promoter through an Estrogen Response Element Hemi-Palindrome Present at -1772bp
Autor/es:
SCHTEINGART HELENA F; VALERI CLARA; EDELSZTEIN NADIA; RIGGIO M; GIULIANELLI S; LANARI CLAUDIA; REY RODOLFO A.
Lugar:
San Diego , California
Reunión:
Congreso; Endocrine Society's 97th Annual Meeting; 2015
Institución organizadora:
The Endocrine Society
Resumen:
AMH is expressed at high levels in prepubertal Sertoli cells of the testis. FSH increases, whereas androgens inhibit, testicular AMH production. In patients with Androgen Insensitivity Syndrome or with Peutz-Jeghers Syndrome, AMH testicular production is increased, concomitantly with estradiol. The AMH promoter contains an estrogen response element hemi-palindrome (½-ERE) at position -1772. In this work we assessed whether estrogens are involved in the increase of the AMH promoter activity in a prepubertal Sertoli cell line, SMAT1. Methods and Results: Reporter assays were performed in SMAT1 cells co-transfected with AMH promoter-luciferase plasmids and expression vectors for human estrogen receptor α (ERα) and/or β (ERβ), and incubated with 17β-estradiol (E2) at concentrations ranging from 10-12 to 10-6 M and/or the antiestrogen ICI 182,780 (10-6 M) for 24 h. Luciferase activity was used to estimate the AMH promoter activity, and results were expressed as % of the respective control (mean ± SEM). One sample t-tests were used to compare the results of the stimulated conditions to a 100% value corresponding to the basal activity. The expression of ERα and ERβ was ascertained in transfected SMAT1 cells by immunofluorescence and Western blotting. Maximum AMH promoter activity in ERα-transfected cells was observed with E2 between 10-10 and 10-8 M; subsequent experiments were performed with E2 10-9 M since it is a physiologic concentration within the testis. In ERα-transfected cells, E2 induced a significant increase of the AMH-3068-1 promoter activity (263.5 ± 12.5%, P