CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Felis catus as a model to study follicle biology in vitro
Autor/es:
ROJO, JL; MUSSE, MP; PELUFFO, MC
Lugar:
Grand Rapids, Michigan
Reunión:
Congreso; 47th SSR Annual Meeting; 2014
Institución organizadora:
Society for the Study of Reproduction
Resumen:
Felis catus as a model to study follicle biology in vitro Julieta L. Rojo, Mariana P. Musse, Marina C. Peluffo Centro de Investigaciones Endocrinológicas "Dr. César Bergadá" (CEDIE),CONICET FEI División de Endocrinología, Hospital de Niños Ricardo Gutiérrez, Gallo 1330. CABA. C1425EFD The domestic cat (Felis catus) is classically defined as an induced ovulator with seasonal polyestrous. The cat is commonly used for the study of oocyte cryopreservation based on its potential to serve as a model species for biomedical research and the conservation of endangered felids. The cat also provides a unique and valuable physiological model to study molecular processes within the preovulatory follicle due to the fact that each animal provides between 3 to 7 preovulatory follicles, naturally selected in an "ovulation-ready" state "waiting" for the LH surge during estrous. Highly conserved reproductive mechanisms between humans and feline species have been recently reported, providing an excellent surrogate for understanding events within the ovary. Thus, the aim of this study was to evaluate the response of isolated feline antral follicles to LH. To test this, ovaries were removed from adult female domestic cats (n=15) during the breeding season at unknown stages of the estrous cycle. Antral follicles larger than 0.5 mm were mechanically dissected from the ovary, measured and individually cultured (n=112) for 12 or 24 hr in the absence (Control group) or presence of recombinant human LH (75 mIU/ml; LH group). Out of the total isolated antral follicles one was a preovulatory follicle (3.5 mm). Thus, results from this follicle were analyzed separately. At the end of the culture period, levels of estradiol (E2) and progesterone (P4) in the media were measured from representative samples from each group and time point (n=72) to evaluate their response to LH. Higher levels of P4 in the LH group (p