CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Estrogen effect on Anti-Mullerian hormone (AMH) promoter activity in a prepubertal Sertoli cell
Autor/es:
VALERI CLARA; RIGGIO M; GIULIANELLI S; LANARI CLAUDIA; SCHTEINGART HELENA F; REY RODOLFO A.
Lugar:
Playa del Carmen
Reunión:
Congreso; XXIV Congreso sociedad Latinoamericana de Endocrinología Pedíatrica; 2014
Institución organizadora:
sociedad Latinoamericana de Endocrinología Pedíatrica
Resumen:
ESTROGEN EFFECT ON ANTI-MÜLLERIAN HORMONE (AMH) PROMOTER ACTIVITY IN A PREPUBERTAL SERTOLI CELL LINE Valeri C*, Edelsztein N*, Riggio M***, Giulianelli S***, Lanari C***, Schteingart H*, Rey RA*, * Centro de Investigaciones Endocrinológicas ?Dr. César Bergadá? (CEDIE), CONICET ? FEI ? División de Endocrinología, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina. **Laboratorio de Carcinogénesis Hormonal, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina. *** Laboratorio de Carcinogénesis Hormonal, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (IBYME-CONICET), Buenos Aires, Argentina. Sertoli cells of the prepubertal testis produce high amounts of AMH. FSH stimulates testicular AMH output, whereas androgens inhibit it at puberty. In patients with androgen insensitivity syndrome or with Peutz-Jeghers syndrome, AMH testicular production is increased, concomitantly with the rise in FSH, testosterone and estradiol. The AMH promoter contains an estrogen response element hemi-palindrome (½-ERE) at position -1772. The aim of this work is to assess whether estrogens are involved in the increase of AMH expression in a prepubertal Sertoli cell line, SMAT1. Methods and Results: Reporter assays were performed using an AMH promoter-luciferase vector transfected to SMAT1 cells. Luciferase activity was used to estimate the AMH promoter activity, and results were expressed as % of the respective control (mean ± SEM). One sample t-tests were used to compare them to a 100% value corresponding to the basal activity; a p