CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression and localization of aquaporins in the primate ovulatory follicle and corpus luteum.
Autor/es:
NAZARETH LORETI ; MELINDA J. MURPHY; STELLA CAMPO; CECILY V BISHOP; RICHARD L. STOUFFER; JON D. HENNEBOLD
Lugar:
Grand Rapids
Reunión:
Congreso; SSR 2014; 2014
Institución organizadora:
Society for the Study of Reproduction
Resumen:
Aquaporins (AQP) are composed of three subfamilies, the prototypical aquaporins (AQP1, 2, 4, 5, 6) that transport water, the aquaglyceroporins (AQP3, 7, 9, 10) that also transport small molecules such as glycerol, as well as the superaquaporins (AQP11 and 12A/B) that differ from the others in structure/function and subcellular localization. Different AQPs were identified in a variety of mammalian tissues including kidney, lung, and skin. However, little is known about their expression in reproductive tissues, including the ovarian follicle and corpus luteum (CL). Therefore, our previously published rhesus monkey transcriptome database was analyzed to identify AQP genes whose mRNA levels change in the naturally-selected dominant follicle after the administration of an ovulatory bolus of human chorionic gonadotropin (hCG), as well as in the CL throughout the luteal phase of the menstrual cycle and following its rescue through the administration of exogenous hCG (i.e., simulated early pregnancy; SEP). AQP gene expression was assessed by DNA array (Affymetrix Rhesus Macaque Total Genome Arrays) and/or quantitative real-time PCR (qPCR), whereas cellular localization was determined by immunohistochemistry (IHC). The single periovulatory follicle was obtained from rhesus macaques at 0, 12, 24, and 36 h post hCG stimulus, with the latter group including those that had not ruptured and those that ovulated (n=4-6/group). CL were collected at early (days 3‐5 post-LH surge), mid (days 6‐8), mid‐late (day 10-12), late (day 14-16), and very-late (days 17-19; menses) stages of the luteal phase (n=4/stage). The effects of hCG on luteal AQP mRNA expression in the rhesus macaque CL during SEP (n=3-4/group) were compared with CL obtained during luteal regression in the non-fecund menstrual cycle. Both AQP1 and AQP11 genes were expressed in the preovulatory follicle and CL. While AQP1 mRNA levels did not change through the periovulatory interval, mRNA levels increased (P