Alterations in serum FSH microheterogeneity in oocyte donors and young infertile women.

LORETI NAZARETH ; VENDITTI ANALIA; DUCATELLI M. EUGENIA ; COCO ROBERTO ; CAMPO STELLA

Tours

Congreso; International Conference on Gonadotropins and receptors; 2014

It was previously demonstrated that the oligosaccharide structure of circulating FSH changes under different physiological conditions in women. There is no available information about the possible impact that multiple stimulation cycles may have on young women hypothalamic-pituitary-ovarian axis function. The aim of the present study was to determine the relative abundance of serum FSH charge analogues as a marker of the hypothalamic-pituitary-ovarian axis function in oocyte donors who had participated in repeated controlled ovarian stimulation (COS) and in young women who had undergone previous unsuccessful infertility treatments. Twenty normal women were included; eight controls aged 20-39; six oocyte donors aged 22-32 who underwent 3 to 8 COS and six infertile women aged 32-39 with a history of unsuccessful outcomes of COS. Blood samples were obtained in the mid-follicular phase (day 9 ± 1) of non-stimulated menstrual cycle before undergoing a new stimulation protocol (oocyte donors and infertile women). FSH, LH, E2, Pro-αC, Inh B and AMH serum levels were determined and the relative abundance of FSH charge analogues was analysed by preparative isoelectic focusing. Elevated gonadotrophins (p