CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Normal and pathological distribution of the perinuclear theca during human spermatogenesis
Autor/es:
CONSTANZA BRANZINI; PETER SUTOVSKY; HERNÁN ELENA; HÉCTOR CHEMES; VANESA RAWE
Lugar:
Baltimore, USA
Reunión:
Congreso; 61 reunion de la American Society of Reproductive Medicine; 2006
Institución organizadora:
American Society of Reproductive Medicine
Resumen:
Normal and pathological distribution of the perinuclear theca during human spermatogenesis Constanza Branzini1, Peter Sutovsky2, Hernán Elena, Héctor Chemes3, Vanesa Rawe1. 1Centro de Estudios en Ginecología y Reproducción, Argentina.2 Departments of Animal Science, Obstetrics and Gynecology, University of Missouri–Columbia, USA.3Laboratorio de Fisiología y Patología Testicular, CEDIE, Hospital de Niños R. Gutierrez, Argentina. Objective: Agregar tres o cuatro renglones de lo que es la globo y cómo se afectan estas estructuras (PT, Actin and acrosome) In the present report we analyze the distribution of the Perinuclear Theca (PT), Acrosome (Acr) and microfilaments (Act) in human ejaculated spermatozoa and testicular spermatogenic cells from fertile individuals and a patient with round-headed acrosomeless spermatozoa (globozoospermia) Materials and methods: Normal and abnormal spermatozoa and immature spermatogenic cells were analyzed using Immunocytochemistry (ICC) and Transmission Electron Microscopy (TEM) of epoxy-embedded material. Monoclonal and polyclonal antibodies reactive against the PT, Acr, and Act were used to examine these cellular structures. Cells were studied under epifluorescence and electron microscopy and images were edited using Adobe Photoshop 7.0. Results: Immature spermatids from fertile individuals showed the development of the acrosomic granule and cap over the cranial pole of the nucleus at all stages of maturation. The PT was always closely associated with the Acr. This association was lost in spermatids from the globozoospermic patient where Acr and PT were located away from  the nucleus with subsequent loss with the residual body. In normal ejaculated spermatozoa the acrosome appeared as a cap covering the anterior 2/3 of the sperm nucleus. As expected, the PT had a subacrosomal and equatorial localization. Ejaculated spermatozoa from the globozoospermic patient presented severe alterations in the pattern of distribution of the PT, Acr and Act, evidenced by the absence or lack of association of the PT and Acr with the apical region of the sperm nucleus. Conclusions: The combined use of phase contrast, epifluorescence and transmission electron microscopy applied to pathological human sperm has allowed us to study in depth the biogenesis of the acrosome, perinuclear theca and actin organization in normal and pathological samples. In our globozoospermic patient the lack of acrosome is not due to lack of development but rather to loss at spermiation of misplaced Acr and PT that develop independiently from the nucleus. These results provide a preliminary indication that proteins of the PT anchor the Acr to the nucleus. The distortions observed with the use of specific antibodies correlated with the ultrastructural findings. The study of the internal organization of the sperm acrosome and the topographical view of whole cells is a perfect combination for a comprehensive understanding of sperm pathologies.