Measurement of inhibin B (InhB) by a newly developed ELISA in the assessment of ovarian function from birth to adulthood

CAMPO S; LORETI N; AMBAO V; GRINSPON R; BEDECARRAS P; ROPELATO MG; BERGADA I; REY R

Glasgow

Congreso; 50th Annual Meeting of European Society for Paediatric Endocrinology (ESPE); 2011

European Society for Paediatric Endocrinology (ESPE)

Background: The stage of follicular development, the degree of granulosa cell differentiation and the concentration of local regulators determine the characteristics of serum InhB pattern in females form birth to adulthood. A third generation of immunoassay has been developed shortening the time of the whole procedure. Objective: Our aim was to establish the normal range of inhB levels using a newly developed assay (Active® Inhibin B Gen II ELISA, Beckman Coulter, USA), in females from birth to puberty and in different stages of the reproductive life. Methods: 97 normal girls, 43 fertile menstruating women and 8 cycling perimenopausal women were included. Serum levels of gonadotrophins, estradiol and inhB were determined. Results: During the first month of life, very low or undetectable inhB levels were present: 9.1±6.3 pg/mL (mean ±SD). A transient increase was observed around day 30 of life; however, these values did not reach the pubertal range (p