CEDIE   05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Insulin-like Growth Factor-1 (IGF-1) Regulates Pheochromocytoma cellular proliferation in vitro and in vivo.
Autor/es:
FERNANDEZ MC; VENARA MC,; NOWICKI S; ALVAREZ SEDÓ, C; CHEMES H E,; BARONTINI M B.; PENNISI PA,
Lugar:
Cartagena de Indias
Reunión:
Congreso; XXII Congreso Sociedad Latinoamericana de Endocrinología Pediátrica (SLEP); 2011
Resumen:
Introduction and Aim.  IGFs are involved in malignant transformation and tumoral growth of the adrenal gland Aim:To evaluate the impact of IGF-1 on Pheochromocytoma?s developmentMaterials and Method.  MPC4/30 cells proliferation, apoptosis, colony formation and migration with or without rhIGF-1 . MPC cells were injected to control and liver-IGF-1-deficient (LID)mice treated with rhIGF-1or vehicle.Results.  MPC proliferation and BrdU incorporation increased significantly upon rhIGF-1 while the percentage of apoptotic cells was significantly lower; migration and soft agar colonies size and number were also stimulated by rhIGF-1 (Table 1). Six weeks after MPC injection all control but only 40% of LIDmice developed tumors. LID harboring MPC and treated with rhIGF-1 developed tumors as controls.The latency period for tumor growth increased in LID as compared to both control and LID+rhIGF-1 [MedianSurvivalRatio:2.2,95%CI1.6-2.8;1.8,95%CI1.4-2.3respectively,p<0.0001]. LID had 10 times lower probability for developing tumors than controls (HR:0.10,95%CI0.02-0.27); rhIGF-1 treatment increased this risk 8 times (HR:7.9,95%CI1.6-67.1)Conclusions.  Cellular proliferation of pheochromocytoma is regulated by IGF-1 in vitro and in vivo. IGF-1 enhanced proliferation, migration and cell´s ability to grow unattached.