FUNCION DE LOS RECEPTORES NOTCH 1 Y 4 EN UNA LINEA DE CELULAS OVÁRICAS TUMORALES HUMANAS (KGN)

PAZOS C; HERNANDEZ F; DE ZÚÑIGA I; ABRAMOVICH D; LORETI N; CAMPO S; TESONE M; IRUSTA G

Ciudad Autónoma de Buenos Aires

Jornada; XII Jornada Anual de la Sociedad Argentina de Biología (SAB); 2010

Introduction: Ovarian cancer involves a type of solid tumours and in spite of having a good response to treatments, it is frequently recurrent. Notch system is an intracellular pathway that regulates cell growth and homeostasis of embryonic and adult cells. Objectives: a.To evaluate the expression of NOTCH 1 and 4 receptors in a tumoral granulosa cell line KGN (human ovarian granulosa) and in human granulosa cells obtained from women subjected to assisted reproductive techniques (ARTs). b. To study the effect of NOTCH 1 and 4 receptors signaling blockade on the proliferation of KGN cell line. Methodology: we performed western blot to analyze the protein content of the NOTCH 1 and 4 receptors in KGN cell line and human granulosa cells obtained from women subjected to assisted reproductive techniques (ARTs). We studied KGN cellular proliferation by 3H-thymidine incorporation in the presence of different concentrations (10-30M) of a Notch pathway inhibitor (DAPT). Results: we observed higher expression for both receptors in KGN cell line compared to granulosa cells obtained from women subjected to ARTs. When KGN cells were cultured in the presence of the inhibitor DAPT, we detected a dose-dependant significant decrease (P