Follicle stimulating hormone and its second messenger cyclic adenosine 5’-monophosphate stimulate the transcription of human anti-Müllerian hormone in cultured granulosa cells

TAIEB, J.; GRYNBERG, M.; PIERRE, A.; AROUCHE, N.; MASSART, P.; BELVILLE, C; HESTERS, L.; FRYDMAN, R.; CATTEAU-JONARD, S.; FANCHIN, R.; PICARD, J.Y.; JOSSO, N; REY, R; DI CLEMENTE, N

MOLECULAR ENDOCRINOLOGY

ENDOCRINE SOC

Año: 2011 vol. 25 p. 645 - 655

0888-8809

Anti-Mu¨ llerian hormone (AMH), also called Mu¨ llerian-inhibiting substance, a member of the TGF-ß family, is responsible for the regression of Mu¨ llerian ducts in the male fetus. In females, AMH is synthesized by granulosa cells of preantral and small antral follicles, and production wanes at later stages of follicle maturation. Using RT-PCR in luteal granulosa cells in primary culture and reporter gene techniques in the KK1 granulosa cell line, we show that FSH and cAMP enhance AMH transcription, and LH has an additive effect. Gonadotropins and cAMP act through protein kinase A and p38 MAPK signaling pathways and involve the GATA binding factor-4 and steroidogenic factor-1 transcription factors, among others. The expression profile ofAMHand the dynamics of serumAMHafter gonadotropin stimulation have been interpreted as a down-regulating effect of FSH upon AMH production by granulosa cells. The specific effect of gonadotropins upon granulosa cells may be obscured in vivo by the effect ofFSHuponfollicularmaturationandbythepresence of otherhormonesandgrowthfactors, acting individually or in concert.in vivo by the effect ofFSHuponfollicularmaturationandbythepresence of otherhormonesandgrowthfactors, acting individually or in concert.