CEDIE 05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
artículos
Título:
Carbohydrate complexity and proportion of serum FSH isoforms reflect pituitary-ovarian activity in perimenopausal women and depot medroxyprogesterone acetate users.
Autor/es:
LORETI N; AMBAOV; JULIATO CT; MACHADO C; BAHAMONDES L; CAMPO S
Revista:
CLINICAL ENDOCRINOLOGY
Editorial:
Blackwell Publishing
Referencias:
Lugar: Oxford, England; Año: 2009 p. 558 - 565
ISSN:
0300-0664
Resumen:
ABSTRACT
BACKGROUND: FSH is synthesized and secreted in multiple glycosylation variants with different oligosaccharide structures; the endocrine milieu regulates the composition of FSH carbohydrate moiety.
OBJECTIVE: To characterize serum FSH isoforms according to their sialic acid content and oligosaccharide complexity in regularly menstruating women and in depot medroxyprogesterone acetate (DMPA) users during the menopausal transition.
SUBJECTS AND METHODS: Ten regularly menstruating perimenopausal women aged 45-52, with mid-follicular phase FSH levels £ 10 IU/l and 10 regularly menstruating women, aged 20-39, were included. Blood samples were collected on the 9th day of the menstrual cycle. Twenty DMPA users were divided into two groups (n=10) according to age: DMPA1, age range 20-39 and DMPA2, age range 45-52. Blood samples were collected 90 ± 5 days after the last injection of DMPA. Oestradiol (E2), inhibin B (Inh B), Pro-áC levels and the relative abundance of FSH isoforms on the basis of charge (preparative isoelectric focusing) and carbohydrate complexity (Concanavalin A chromatography) were determined.
RESULTS: Decreased Inh B and moderately elevated E2 levels were observed in perimenopausal women associated with an increase in FSH sialylation and a decrease in its oligosaccharide complexity. DMPA induced changes in the hormonal profile and FSH molecular microheterogeneity; the secreted hormone was more heterogeneous and its oligosaccharides were less complex under this condition.
CONCLUSIONS: Serum FSH glycoforms with increased sialylation and decreased oligosaccharide complexity reflect the decline of the gonadal activity induced either by age or by the use of a DMPA as a contraceptive.
