Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism.

YAKAR, SHOSHANA; ROSEN, CLIFFORD J; BOUXSEIN, MARY L; SUN, HUI; MEJIA, WILSON; KAWASHIMA, YUKI; WU, YINGJIE; EMERTON, KELLY; WILLIAMS, VALERIE; JEPSEN, KARL; SCHAFFLER, MITCHELL B; MAJESKA, ROBERT J; GAVRILOVA, OKSANA; GUTIERREZ, MARIANA; HWANG, DAVID; PENNISI, PATRICIA; FRYSTYK, JAN; BOISCLAIR, YVES; PINTAR, JOHN; JASPER, HECTOR; DOMENÉ, HORACIO; COHEN, PINCHAS; CLEMMONS, DAVID; LEROITH, DEREK

FASEB JOURNAL

Federation of American Societies for Experimental Biology

Lugar: Bethesda, Maryland, USA; Año: 2009 vol. 23 p. 709 - 719

0892-6638

Serum insulin-like growth factor (IGF) -1 is secreted mainly by the liver and circulates bound to IGF-binding proteins (IGFBPs), either as binary complexes or ternary complexes with IGFBP-3 or IGFBP-5 and an acid-labile subunit (ALS). The purpose of this study was to genetically dissect the role of IGF-1 circulatory complexes in somatic growth, skeletal integrity, and metabolism. Phenotypic comparisons of controls and four mouse lines with genetic IGF-1 deficits— liver-specific IGF-1 deficiency (LID), ALS knockout (ALSKO), IGFBP-3 (BP3) knockout, and a triply deficient LID/ALSKO/BP3 line—produced several novel findings. 1) All deficient strains had decreased serum IGF-1 levels, but this neither predicted growth potential or skeletal integrity nor defined growth hormone secretion or metabolic abnormalities. 2) IGF-1 deficiency affected development of both cortical and trabecular bone differently, effects apparently dependent on the presence of different circulating IGF-1 complexes.1) All deficient strains had decreased serum IGF-1 levels, but this neither predicted growth potential or skeletal integrity nor defined growth hormone secretion or metabolic abnormalities. 2) IGF-1 deficiency affected development of both cortical and trabecular bone differently, effects apparently dependent on the presence of different circulating IGF-1 complexes.2) IGF-1 deficiency affected development of both cortical and trabecular bone differently, effects apparently dependent on the presence of different circulating IGF-1 complexes. 3) IGFBP-3 deficiency resulted in increased linear growth. In summary, each IGF-1 complex constituent appears to play a distinct role in determining skeletal phenotype, with different effects on cortical and trabecular bone compartments.—) IGFBP-3 deficiency resulted in increased linear growth. In summary, each IGF-1 complex constituent appears to play a distinct role in determining skeletal phenotype, with different effects on cortical and trabecular bone compartments.—