CEDIE 05498
CENTRO DE INVESTIGACIONES ENDOCRINOLOGICAS "DR. CESAR BERGADA"
Unidad Ejecutora - UE
artículos
Título:
Assessing Sertoli Cell Metabolic Activity
Autor/es:
OLIVEIRA, PEDRO F.; GALARDO, MARÍA N.; ALVES, MARCO G.; RINDONE, GUSTAVO; RIERA, MARÍA F.; JARAK, IVANA; CARVALHO, RUI A.; MERONI, SILVINA B.
Revista:
METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)
Editorial:
Humana Press
Referencias:
Lugar: Clifton NJ; Año: 2018 vol. 1748 p. 157 - 171
ISSN:
1064-3745
Resumen:
Nuclear magnetic resonance (NMR)-based metabolomics is widely used in the research of metabolic conditions of complex biological systems under various conditions, and its use has been found in the field of male fertility. Here we describe the implementation of total and targeted NMR-based metabolomics in the research on Sertoli cell metabolism. Main principles and techniques of cell medium, cellular extracts, and intact cells are explained, as well as some classical experiments that can give complementary information on the Sertoli cell metabolism.
