Carbohydrate complexity and proportions of serum FSH isoforms

S. CAMPO; V. AMBAO; S. CREUS; S. GOTTLIEB; G. FERNANDEZ VERA; H. BENENCIA; C. BERGADA

MOLECULAR AND CELLULAR ENDOCRINOLOGY.

Elsevier Ireland Limited

Año: 2007 vol. 260 p. 197 - 204

0303-7207

Abstract FSH is synthesized and secreted in multiple molecular forms with different oligosaccharide structures which are needed for full expression of biological activity. GnRH and sex steroids modulate oligosaccharide structure and composition. In the present study we have assessed the carbohydrate complexity and proportion of circulating FSH isoforms during puberty, aging and after androgen administration to pubertal anorchid boys. Preparative isoelectrofocusing and lectin chromatography were used to isolate FSH isoforms on the basis of charge and internal carbohydrate complexity. Differences in sialic acid content and a progressive increase of isoforms bearing highly branched oligosaccharides were found during puberty. Less acidic, more bioactive FSH isoforms, secreted at mid-puberty may modulate important maturational events in the Sertoli cell population. Androgen administration to pubertal anorchid boys favoured the secretion of this type of isoforms. In adult men, the predominance of FSH isoforms bearing complex type oligosaccharides remained unchanged until very advanced age. These results show that the predominance of FSH isoforms bearing fully processed oligosaccharides in circulation may contribute to the development and maintenance of seminiferous epithelium function in men. © 2006 Elsevier Ireland Ltd. All rights reserved