Cut-off values of serum growth hormone (GH) in pharmacological stimulation tests (PhT) evaluated in short-statured children using a chemiluminescent immunometric assay (ICMA) calibrated with the International Recombinant Human GH Standard 98/574

CHALER EA; BALLERINI G; LAZZATI JM; MACEIRAS JM; FRUSTI M; BERGADÁ I; RIVAROLA MA ; BELGOROSKI A; ROPELATO G

CLINICAL CHEMISTRY AND LABORATORY MEDICINE

WALTER DE GRUYTER & CO

Lugar: Berlin; Año: 2013 vol. 51 p. 95 - 97

1434-6621

The diagnosis of growth hormone deficiency (GHD) is based primarily on auxological criteria. Diagnosis is confirmed biochemically by the assessment of GH during pharmacological tests (PhT). We proposed that the cut-off diagnostic limit of serum GH in PhT should be defined for each immunoassay [1, 2] . The first International Standard (IS) used in immunoassays was International Reference Preparation (IRP) 66/217 in 1969. It was a preparation from human GH containing various hGH molecular forms, In 1982 the WHO proposed the IRP 80/505 for use in bioassays. In 1987 this preparation from human GH began to be used in immunoassays and in 1992 it was calibrated to units and milligrams (rDNA-derived GH). In 1994 WHO prepared the first IS IRP 88/624 (rhGH) and in 1998 the second IS IRP 98/574 (rhGH) was proposed for harmonization of GH results in immunoassays.