Standarization of a multi-specie blocking elisa to assess specificity and avidity of antibodies against bovine

FRANCO, OLGA; COMBESSIES, GUSTAVO; OGAS, LORENA; SEKI, CRISTINA; ROBIOLO, BLANCA; LA TORRE, JOSE; GRIGERA, PABLO; CAPOZZO, ALEJANDRA

Madrid, España

Simposio; World Association of Veterinary Laboratory Diagnosticians- 14th International Symposium; 2009

The most reliable technique to assess immune responses against BVDV is seroneutralization assay (SN). However, this test is laborious to perform and entails the risk of live virus manipulation. In order to have an alternative in vitro technique to the SN assay, we developed a multi-specie blocking ELISA (B-ELISA) together with an avidity ELISA (AB -ELISA) using a truncated Glycoprotein E2 produced by recombinant baculovirus, for titration of antibodies to BVDV. E2 is the most immunogenic protein of BVDV and elicits high titers of neutralizing antibodies after infection.in vitro technique to the SN assay, we developed a multi-specie blocking ELISA (B-ELISA) together with an avidity ELISA (AB -ELISA) using a truncated Glycoprotein E2 produced by recombinant baculovirus, for titration of antibodies to BVDV. E2 is the most immunogenic protein of BVDV and elicits high titers of neutralizing antibodies after infection.