Effects of oligodeoxynucleotide imt504 on cell viability and gene expresion in a murine beta cell line (MIN6B1)

LIBERTUN, CARLOS; CONVERTI, AYELÉN; LUX-LANTOS, VICTORIA; MONTANER, ALEJANDRO; BONAVENTURA, MARÍA MARTA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

EFFECTS OF OLIGODEOXYNUCLEOTIDE IMT504 ON CELL VIABILITY AND GENE EXPRESION IN A MURINE BETA CELL LINE (MIN6B1)We have previously demonstrated that treatment with IMT504 promotes significant improvement in the diabetic condition in diverse animal models. We have also shown effects on gene expression on freshly isolated islets from diabetic IMT504-treated animals. Based on these results, here we evaluated if the effects of IMT504 observed in vivo were due to direct effects on beta cell function. In particular we studied cell viability and gene expression. A murine beta cell line (MIN6B1, kindly donated by Dr. P. Halban) was used. Cells were cultured in DMEM with 20 mM glucose, 15% SFB, 71 uM βmercaptoethanol. Cell viability was analized by MTS while gene expression of Pdx-1, INS2, INS1 and MafA was analized by qPCR, using cyclophilin as housekeeping gene. To study cell viability, cells were stimulated for 24 or 48 h with 0 (C) 0,4 (IMT0,4), 2,2 (IMT2,2) and 4 ug/ul (IMT4) of IMT504 in DMEM, 20 mM glucose, 0,5% BSA, 71 uM βmercaptoethanol. For gene expression, cells were stimulated for 12, 24 and 48 h with the same stimuli. No differences in cell viability were observed at the time points studied (ANOVA for repeated measures: NS; n=5). Expression of Pdx-1 and INS2 was significantly increased by 48 h stimulation with IMT504 [ANOVA for repeated measures: Pdx-1 (A.U.): C=0,47±0,03; IMT0,4=0,40±0,03; IMT2,2=0,52±0,04; IMT4=0,68±0,05 p