Use of Monoclonal Antibodies for Rapid Characterization of Field Oubreaks of FMDV

SEKI, C.; ROBIOLO, B.; BERGMANN, I.; MALIRAT, V.; PEREZ, C.; PERIOLO, O.; MATTION, N.; MARADEI, E.; LA TORRE, J.

Berlin

Simposio; 10th OIE Seminar 32nd Symposium of AVID; 2013

16th Intern. Symposium of the World Association of Veterinary Laboratory Diagnosticians

Purpose Panels of monoclonal antibodies (MAbs) developed against different foot-and-mouth disease virus (FMDV) vaccine strains previously characterized were used for in process control and evaluation of commercial vaccines. Here we described a rapid method to analyze the evolution of field strains in viral outbreaks occurred in South America. Methods The reactivity with reference strains and field isolates was evaluated by ELISA using type specific MAbs. Linear antigenic profiles or checkerboard graphs were used to compare different isolates. Correlation coefficients of reactivity were used for the comparison of antigenic profiles. Results The screening of FMDV field isolates from the 2000?2001 outbreaks in Argentina based on these correlation coefficients was capable to identify two main groups of FMDV isolates and the transient presence of other minor variants. During the 2009-2010 outbreaks in Ecuador, a panel of 20 MAbs (raised against O Caseros, O1Campos and O Taiwan) was used to analyze type O viruses. MAbs? profiling grouped the isolates into two major reactivity patterns which differed from that of the vaccine strain. The same type O panel of MAbs was used to analyze isolates from FMDV outbreaks in Paraguay. Marked differences with the vaccine strain O1/Campos were recognized, including the loss of reactivity with some neutralizing MAbs. Conclusions ELISA carried out using panels of MAbs allows a rapid analysis of strains that are circulating during outbreaks and helps to determine which isolates are epidemiologically relevant to be considered for further studies, such as sequencing, cross protection by neutralization tests, lp-ELISA, and eventually virus challenge in susceptible animals.