Influence of the KDEL signal, DMSO and mannitol on the production of the recombinant antibody 14D9 by long-term Nicotiana tabacum cell suspension culture

LÓPEZ J, LENCINA F, PETRUCCELLI S, MARCONI P, ALVAREZ MA

PLANT CELL TISSUE AND ORGAN CULTURE

SPRINGER

Año: 2010 vol. 103 p. 307 - 314

0167-6857

We have established two transgenic cell suspension culture lines of Nicotiana tabacum that express the catalytic antibody 14D9 as a secretory product (sec-Ab) or as a KDEL-tagged product in the endoplasmic reticulum (Ab-KDEL), respectively. After 3 years of culture, the performance improved to a production level of 0.15 ± 0.03 lg ml-1 on the seventh day of culture for the sec-Ab line and 0.48 ± 0.05 lg ml-1 on the third day for Ab-KDEL line. Analysis of the effect of osmotic stress using mannitol (90 g l-1) as an osmolite revealed that there was a 12-fold increase in antibody yield (1.96 ± 0.20 lg ml-1) on the seventh day of culture in line sec-Ab and a fivefold increase (2.31 ± 0.18 lg ml-1) on the seventh day for line Ab- KDEL. The concentration of the antibody in the culture medium was not significant. Dimethyl sulfoxide used as a permeabilizing agent was not effective in increasing 14D9 yield, but it did cause distinctive cell damage at all concen trations tested.