CONICET | Buscador de Institutos y Recursos Humanos

cDNA array technology was used to compare transcriptome profiles of Lotus japonicus roots inoculated with a Mesorhizobium loti wild-type and two mutant strains affected in cyclic (1-2) glucan synthesis or in lipopolysaccharide synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. mutant strains affected in cyclic (1-2) glucan synthesis or in lipopolysaccharide synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. Lotus japonicus roots inoculated with a Mesorhizobium loti wild-type and two mutant strains affected in cyclic (1-2) glucan synthesis or in lipopolysaccharide synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. (1-2) glucan synthesis or in lipopolysaccharide synthesis. Expression of genes associated with the development of a fully functional nodule was significantly affected in plants inoculated with the glucan mutant. Array results also revealed that most of the genes normally induced during nodule development suffered a delay of induction in roots that were inoculated with the lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. lps mutant. Quantitative Real time RT-PCR was used to quantify gene expression of a subset of genes involved in plant defenseresponss, or redox metabolism and/or nodulins. The majority of the genes analyzed in this study were more highly expressed in roots inoculated with the wild type compared to those inoculated with the glucan mutant strain. Some of the genes exhibited a transient increased in transcript levels during intermediate steps of normal nodule development, while others displayed induced expression during the final steps of nodule development. Ineffective nodules induced by the glucan mutant showed higher expression of phenylalanine ammonia lyase than wild-type nodules. Differences in nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan. nin expression pattern were observed in plants inoculated with M. loti mutant strain affected in the synthesis of cyclic glucan.M. loti mutant strain affected in the synthesis of cyclic glucan.