Establishment of Chia (Salvia hispanica, Labiateae) in vitro plant and cell cultures

M ALEJANDRA ALVAREZ, PATRICIA LAURA MARCONI

Biotecnología Vegetal

Instituto de Biotecnología de las Plantas, Universidad Central Marta Abreu de Las Villas

Lugar: Santa Clara; Año: 2013 vol. 13 p. 1 - 6

2074-8647

We have optimized the culture conditions for the initiation and maintenance of Salvia hispanica L. (Chia) callus cultures. Stems were the best explant source for starting in vitro cultures. Both IAA and NAA, at all the concentration tested with or without the addition of citokinines, have induced the development of adventitious roots both in darkness as in light. In darkness, 2,4-D (2.25 mM) has induced the development of S. hispanica embryogenic tissues and in light promoted the initiation and establishment of green, and fast-growing calli that lost their friability with time. When subcultured to MS culture media with NAA:kin (0.54 mM:0.46 mM) or Picloram (1.72 mM, 3.43 mM) as plant growth regulators, calli maintained friability for two years in culture. As for the fat content of the calli obtained, values remained significant lower than those of seeds.