Liposomal vehiculization of Zn-phtahlocyanines and amine derivates in PDT inactivation of T98g cells

MIRETTI, M; TEMPESTI, T. C; CAPUTTO, BL; PRUCCA, CG; VELAZQUEZ, FN; BAUMGARTNER, M. T

Congreso; LII Reunión SAIB 2016; 2016

Photodynamic therapy (PDT) is considered a promising strategy for cancer treatment. Phthalocyanines (Pcs) are good photosensitizers(PS) for PDT because they induce cell death in several cellular models. The lipophilic nature of many PS may be an important factoraffecting their preferential accumulation in tissues. However, due to their hydrophobicity, intravenous treatment is greatly hampered.Liposomal preparations are currently used as an effective delivery system in experimental studies and clinical trials. Several reportshave shown the advantages of liposomal preparations over other formulations. The PS incorporation into liposomes assures theirmaintenance in a monomeric state. The aim of this work was to evaluate the properties of two Pcs (ZnPc and TAZnPc) vehiculizedinto DPPC-cholesterol liposomes for PDT on glioblastoma cells. All the Pcs were innocuous in absence of light at concentrations ≤ 0,5µM. However, after irradiation, both formulations induce cell death in a concentration and light dose dependent manner. The averagesize of the resulting liposomes was 112.5 ± 1.35 nm for ZnPc and 228.1 ± 12.3 nm for TAZnPc. Both Pcs show an increase in theirphotosensitizing activity when they were administrated in DPPC-cholesterol liposomes rather than in DMF. Moreover, we observed arelation between liposomal size and cellular death induction.