Fluorescent biodegradable core-shell Nanoparticles applied as platforms for molecular diagnosis by Nanoimaging and drug delivery applications

DENIS BOUDREAU; A. G. BRACAMONTE; D. GONTERO; ALICIA V. VEGLIA

Simposio; Molecular Diagnostics 2016. Virtual Event. Labroots, ACS; 2016

The pharmaceutical drugs are used in many formulations, dosifications depending of the health problem. Many of them can generate difficulties for an optimal treatment due to their biodisponibility, frequency of administration or toxicity. For this reason the application of Biodegradables Nanoparticles chemically modified permits the improving of the availability of the pharmacophore in a specific site by molecular targeting, and by this way, diminishing non desirable secondary reactions. In the present work it is proposed the generation of Biodegradable core-shell Nanoparticles with the following characteristics: a) A metallic core-shell that it is a platform for chemical modification, a cargo vehicle and with intrinsic photothermic properties. b) The biodegradable polymer permits the drug delivery and a fluorophore incorporation for their detection. c) The Nanoparticle surface permits the Bioconjugation of biological molecules and antibodies for molecular and cellular targeting.The gold Nanoparticles synthesis was done with the classical Turkevitch method that it consists in the reduction reaction of Au+3 to Au0 with citrate. [ ] The biodegradable polymer is a copolymer of lactic and malic acid. The synthesis was done by the Spontaneous emulsification solvent diffusion method.[ ] For the synthesis of Biodegradable Core-shell Nanoparticles were assayed different colloidal dispersion and monomers concentrations. The optimal experimental conditions shown, by TEM images, a 20 nm metallic core covered with a 18 nm polymeric shell. This Nanostructure was chemically modified with Lucifer-Yellow modeling the drug delivery experiments and by this manner permitted the quantification of the drug loaded. By fluorescence was quantified a maximal concentration delivered of 18 nM in 168 h. period of time in buffer phosphate pH=7,00. By Nanoimaging with fluorescence microscopy, clear and brights luminescent host spots were observed. On a next stept it will be chemically modified the shell surface to cell targeting for oncology applications.