INFIQC   05475
INSTITUTO DE INVESTIGACIONES EN FISICO- QUIMICA DE CORDOBA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Electrochemical (bio)sensors based on the use of carbon nanomaterials
Autor/es:
EMILIANO N. PRIMO; GUSTAVO A. RIVAS; FABIANA A. GUTIERREZ; AURELIÉN GASSNIER; MARCOS EGUILAZ RUBIO; ALEJANDRO GUTIÉRREZ; GUILLERMINA L. LUQUE; MARÍA L. PEDANO; MARCELA C. RODRIGUEZ; NANCY F. FERREYRA; MARÍA D. RUBIANES
Lugar:
Angra dos reis
Reunión:
Congreso; 16th Tropical meeting of the International Society of the Electrochemistry; 2015
Institución organizadora:
International Society of the Electrochemistry
Resumen:
This presentation is focused on the discussion of new strategies for the design of (bio)electroanalyticalplatforms devoted to the sensitive and selective quantification of biomarkers based on the use of carbonnanomaterials. Special attention will be given to the critical selection of the molecules used tofunctionalize the carbon nanostructures in order to obtain nanomaterials with biorecognition,preconcentration or electrical properties that make possible the efficient sensing of important bioanalytes.We discuss the advantages of electrochemical (bio)sensors obtained by modification of glassy carbonelectrodes (GCE) with multiwalled carbon nanotubes (MWCNT) non-covalently functionalized bydispersion in polyhistidine (Polyhis), calf-thymus double stranded DNA (dsDNA), and polylysine (Plys).The dispersions were obtained by mixing the MWCNT with the given polymer (prepared in 50% ethanolsolution) followed by sonication for a given time. The (bio)sensors were obtained by drop-coating onpolished GCE and further evaporation of the solvent at room temperature. The resulting electrodes(GCE/CNT-polymer) were used for (bio)sensing of : I) the hybridization event using GCE/CNT-Plystaking the system oligo(dG)-oligo(dC) as model; II) glucose using GCE/CNT-polymer modified by self-assembling of glucose oxidase (GOx); III) Cu(II) using GCE/CNT-Polyhis; IV)promethazine usingGCE/MWCNT-dsDNA. The modification of GCE with SWCNTs covalently functionalized with Polylys,lysine, tyrosine, and cysteine was also investigated to obtain analytical platforms for the quantification ofglucose, dopamine and heavy metals, respectively.Chemically reduced graphene oxide obtained from the oxidation of graphite and graphene nanoribonsobtained from the oxidation of MWCNTs were also used for the construction of (bio)sensingelectrochemical platforms. The reduced graphene oxide (RGO) was incorporated in a composite materialcontaining graphite and mineral oil and the resulting electrode was used for the successful quantificationof NADH and Do in the presence of ascorbic acid. The usefulness of graphene nanoribons was alsodemonstrated in connection with the highly successful quantification of dopamine either in solution or byadsorptive stripping with differential pulse voltammetry transduction and medium exchange.In summary, the proposed (bio)sensors demonstrate that the judicious and intimate coupling of a suitable(bio)molecule and an electrochemical transducer efficiently modified with carbon nanomaterials makespossible the development of highly sensitive and selective bioanalytical devices.