Surface bio-functionalization to improve the performance of biomaterials

DARIANA ARISTIZABAL BEDOYA; MARIA LAURA MARTIN; MARIA FERNANDA STRAGLIOTTO; CECILIA VASTI; LAURA E. VALENTI; RICARDO ROJAS; CARLA E. GIACOMELLI

Amsterdam

Congreso; 5th International Colloids Conference; 2015

Surface bio-functionalization is of paramount in order to improve the recognition capabilities, the colloidal stability, and the bio-activity to biomaterials. For instance, surface bio-functionalization of drug (nano)carriers is mandatory to induce site-specificity and selectivity to the delivery process, to enhance the colloidal stability in biological relevant media, to tune the particle size distribution, and to get better storage-reconstitution cycles. In the case of prosthesis and implants, surface bio-functionalization increases the biocompatibility and the antibacterial activity of the material.This work is aimed at discussing two experimental systems as a proof of concept of the benefits of surface bio-functionalization. Bovine serum albumin (BSA) was used to improve the particle size distribution, colloidal stability, and reconstitution of biocompatible double layered hydroxides (LDH) and silver nanoparticles (Ag-NP). With such a purpose, the systems were studied using different techniques, like electron microscopy, X-ray diffraction, surface enhanced Raman spectroscopy, dynamic light scattering and electrophoretic mobilities in different experimental conditions (synthesis route, capping agents, pH, ionic strength).BSA behaves as an excellent stabilizing agent when used during the synthesis or after it, controlling the particle size distribution and colloidal stability, especially when nanoparticles were in contact with medium of high ionic strength, as culture and extracellular media. The synthesis route determined the nature of the interaction between BSA and the (nano)particles: 1. BSA incorporation to LDH matrixes containing chloride or amphiphilic anions produced nanohybrids with tunable size and increased colloidal stability, indicating that the protein may be applied to nanocarriers loaded with drugs featuring different physicochemical properties. 2. BSA used during the Ag-NP synthesis produced extremely efficient antimicrobial agent with bactericidal effect in the nanomolar range. The powdered biocidal agent is stable under storage and versatile, as it can be used in different forms.