PHOTODYNAMIC INACTIVATION OF GLIOBLASTOMA CELLS (T98G) USING ZNPC DERIVATES

PRUCCA CG; VELAZQUEZ FN; MIRETTI, M; BAUMGARTNER MT; TEMPESTI T; CAPUTTO BL

Mar del Plata

Congreso; LI Reunion Anual del Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2015

SAIB

The development of novel therapeutic strategies for the treatment of tumors of the CNS is highly important.Glioblastomas are very aggressive brain tumors with poor prognostic and a very short survival period ofapproximately 1 year. The standard protocol for the treatment of these tumors include reductive surgery followed byradio and chemotherapy. Photodynamic therapy (PDT) has been studied for decades and several photosensitizers (PS)were synthetized and shown to be effective, in combination with light, to induce cell death to different tumor cells.Pthalocyanines are molecules with good properties as PS for PDT. The aim of this work was to evaluate theproperties of Zinc pthalocyanines (ZnPc) and the derivates TAZnPc and TMAZnPc as PS for PDT in glioblastomacells (T98G). All the PS were innocuous in absence of light at concentrations 􀂔 0,5 μM. However, after irradiation,both ZnPc and TAZnPc induce cell death in a concentration and light dose dependent manner. Both PS rapidlyinduces phosphatidylserine surface exposure, mitochondrial membrane depolarization, DNA fragmentation andactivation of caspase -3, hallmarks of apoptosis. Nevertheless, ZnPc induces apoptosis with lower doses of light incomparison to TAZnPc. These results suggest that both, ZnPc and TAZnPc are good PS to continue in vivo studies ina Glioblastoma animal model.